Isolation and Identification of γ- Sitosterol by GC-MS from the Leaves of Girardinia heterophylla (Decne)

Nisha Tripathi1, *, Sunita Kumar1, Rakesh Singh2, C.J. Singh3, Prashant Singh4, V.K. Varshney5
1 Department of Chemistry, M.K.P.P.G. College, Dehradun, Uttarakhand, India;
2 Department of Chemistry, D.B.S.P.G. College, Dehradun, Uttarakhand, India;
3 Forest Conservation Division, Ministry of Environment and Forest, Delhi, India;
4 Department of Chemistry, D.A.V.P.G. College, Dehradun, Uttarakhand, India;
5 Chemistry Division, Forest Research Institute (FRI), Dehradun, Uttarakhand, India

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© Tripathi et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Chemistry, M.K.P.P.G. College, Dehradun, Uttarakhand, India; Mobile: 919873495609; Fax: 0135- 2741147; E-mail:


Girardinia heterophylla, known as Himalayan Nettle commonly grows in temperate and subtropical Himalayas. Plant leaves were collected in winter season and analyzed using appropriate chemical methods and the biochemical constituents were ascertained. An unknown compound was isolated from leaves of the plant. After being collected, analyzed by GC-MS and compared with NIST standard chart library, it was declared to be γ-sitosterol, an epimer of β-sitosterol. γ-sitosterol has been reported for the first time in Girardinia heterophylla and has potential to be used as an antidiabetic owing to its remarkable medicinal properties.

Keywords: : Girardinia heterophylla, GC-MS, γ-sitosterol, β-sitosterol.