RESEARCH ARTICLE


Justification of Traditional Uses of Asparagus Racemosus (Shatavari) - A Miracle Herb



Samira Karim, Md. Shariful Islam, Zarin Tasnim, Farhina R. Laboni, Azima S. Julie, Zubair K. Labu*
Department of Pharmacy, World University of Bangladesh, 151/8 Green road, Dhaka 1205, Bangladesh


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© 2017 Karim et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Pharmacy, World University of Bangladesh, 151/8 Green road, Dhaka 1205, Bangladesh, Tel: 01558388956; E-mail: zubair.labu@yahoo.com


Abstract

Objective:

The ethanolic plant extracts of Asparagus racemosus and their partitionates were assessed for thrombolytic, membrane stabilizing, antimicrobial and antioxidant activity in vitro.

Methods:

The collected plant was dried and grinded. The coarse powder was shocked in 2000 mL of 90% methanol for several days then filtrated. At 40°C the volume of crude ethanolic extract (CEE) was reduced by a vacuum rotary evaporator followed by concentrated aqueous ethanol extractive was fractionated into petroleum ether (PSF), carbon tetrachloride (CTSF), chloroform soluble fraction (CSF) and aqueous (AQSF) soluble fractions.

Results:

In thrombolytic assay among all the extractives, carbon tetrachloride soluble fraction (CTSF) showed the highest clot lysis (41.48 ± 0.43%) activity as compared with standard streptokinase (65.16 ± 0.16%). With respect to the membrane stabilizing activity, chloroform soluble fraction (CSF) profoundly inhibited the lysis of erythrocytes (80.86 ± 0.36%) induced by osmosis, whereas reference standard acetyl salicylic acid (ASA) resulted in (88.99 ± 0.39%) inhibition of lysis. On the other hand, CTSF showed slightly higher level of protection against heat-induced hemolysis (85.68 ± 0.75%) as same as with ASA, (85.68 ± 0.75%)). In antimicrobial investigation, crude ethanol extract (CEE) and their partitioning fractions exhibited moderate inhibition of growth of some examined bacteria. The total phenolic content was found maximum (930.94 mg of GAE/gm) in CEE of A. racemosus. The antioxidant potentials in term of DPPH free radical scavenging assay showed EC50 values 2.62 μg/mL by aqueous soluble fraction (AQSF) in comparison to ascorbic acid 2.48 μg/mL as a reference standard.

Conclusions:

This study was conducted to validate the A. racemosus plant as thrombolytic, membrane stabilizing agent, antimicrobial and antioxidant.

Keywords: Asparagus racemosus, Thrombolytic, Membrane stabilizing, Antimicrobial, Antioxidant activity.